High productivity expression technologies
The broad spectrum of microbial and yeast hosts at Boehringer Ingelheim allows the selection of the most suitable production system in terms of economy, product quality and efficiency.
High-performance expression of peptides and proteins
E.coli: For high-performance expression of peptides and proteins in inclusion bodies, titers up to 15 g/L are achieved. This is four-fold higher than the current industrial standard.
Pichia pastoris: For the methylotroph pichia pastoris explosion-proof fermentation plants are in place. The productivity for Fab fragments is in the range of 5g/L.
Hansenula polymorpha yeast strains: Boehringer Ingelheim RCV GmbH & Co KG has access to high-titer hansenula polymorpha yeast strains with titers above 5g/L.
Saccharomyces cerevisiae fermentation technology is established with a productivity of more than 3g/L.
Setting the Standard for pDNA Production
Boehringer Ingelheim RCV GmbH & Co KG is one of the world's leading contract manufacturers for non-viral pDNA produced by microbial fermentation. With an innovative and strictly quality-driven process design, we provide lot sizes from multi-gram and kilogram scale pDNA.
pDNA upstream processing
- High plasmid DNA fermentation titer up to 2.5 g/L
- Proprietary E.coli host strain ensuring high supercoiled fraction in fermentation
- Antibiotic-free selection system (resistance-free plasmid)
- Proprietary high-cell-density cultivation process up to 6,000 L
- Custom-designed solutions for genetic host / vector engineering
- No raw materials of animal origin, no antibiotics
pDNA downstream processing
- Proprietary and automated cell-lysis system
- Maintenance of supercoiled plasmid conformation (up to 98 %)
- Innovative three-step chromatographic purification
- No detergents, organic solvents and enzymes
